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Diversidade genética entre híbridos de tangerina 'Cravo' e laranja 'Pêra' PAB
Oliveira,Roberto Pedroso de; Cristofani,Mariângela; Aguilar-Vildoso,Carlos Ivan; Machado,Marcos Antônio.
Marcadores moleculares RAPD foram utilizados para avaliar a diversidade genética em uma população de 94 híbridos de tangerina 'Cravo' (Citrus reticulata Blanco) com laranja 'Pêra' (C. sinensis (L.) Osbeck), obtidos por polinização controlada. Nas reações de amplificação foram usados 102 "primers" decâmeros de seqüência arbitrária, que amplificaram 640 fragmentos, sendo 77,2% monomórficos entre os parentais. Utilizou-se o coeficiente de Jaccard para estimar a similaridade genética entre os híbridos, o método UPGMA para gerar o fenograma (NTSYS 1,7) e o software BOOD para determinar a consistência de cada agrupamento. Verificou-se que a laranja 'Pêra' apresenta maior heterozigosidade que a tangerina 'Cravo', sendo, respectivamente, 180 e 126 os números...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Frutas cítricas; Marcadores genéticos; Heterozigoto; Variação genética.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002000400008
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Genetic divergence among hybrids of 'Cravo' mandarin with 'Pêra' sweet orange Scientia Agricola
Oliveira,Roberto Pedroso de; Aguilar-Vildoso,Carlos Ivan; Machado,Marcos Antônio.
Molecular markers have been used as tools in breading programs of sexual hybridation, allowing the genetic characterization of a large number of genotypes. The RADP markers are the most used since the employed techniques are simple and of low cost. To evaluate the genetic divergence among F1 hybrids of 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck), this study analyses the variability and similarity of the hybrids among themselves and with their parents. Random Amplified Polimorfic DNA marker analysis, with 102 primers, were applied to a population composed of 94 hybrids and their parents. Multivariate genetic divergence analysis of the principal components and Tocher grouping were carried out only considering...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus reticulata; Citrus sinensis; Tocher grouping; RAPD; Citrus.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000100017
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Genetic variability in the endophytic fungus Guignardia citricarpa isolated from citrus plants Genet. Mol. Biol.
Glienke-Blanco,Chirlei; Aguilar-Vildoso,Carlos Ivan; Vieira,Maria Lúcia Carneiro; Barroso,Paulo Augusto Vianna; Azevedo,João Lúcio.
During some phases of of their life-cycle endophytic fungi colonize plants asymptomatically being found most frequently inside the aerial part of plant tissues. After surface disinfection of apparently healthy leaves from three varieties of mandarin orange and one tangor, and after incubation on appropriate culture medium, 407 fungal isolates were obtained, giving a total infection frequency of 81%. No fungal growth was observed from disinfected seeds, indicating that fungi are probably not transmitted via seeds. Of the fungal isolates, 27% belonged to the genus Guignardia, with 12 isolates being identified as Guignardia citricarpa Kiely, which is described as a citrus pathogen. The isolates were variable in respect to the presence of sexual structures and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus; Endophytes; Genetic variability; Guignardia citricarpa; Mandarin; RAPD.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200021
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Selection processes in a citrus hybrid population using RAPD markers PAB
Oliveira,Roberto Pedroso de; Aguilar-Vildoso,Carlos Ivan; Machado,Marcos Antônio.
The objective of this work was to evaluate the processes of selection in a citrus hybrid population using segregation analysis of RAPD markers. The segregation of 123 RAPD markers between 'Cravo' mandarin (Citrus reticulata Blanco) and 'Pêra' sweet orange (C. sinensis (L.) Osbeck) was analysed in a F1 progeny of 94 hybrids. Genetic composition, diversity, heterozygosity, differences in chromosomal structure and the presence of deleterious recessive genes are discussed based on the segregation ratios obtained. A high percentage of markers had a skeweness of the 1:1 expected segregation ratio in the F1 population. Many markers showed a 3:1 segregation ratio in both varieties and 1:3 in 'Pêra' sweet orange, probably due to directional selection processes. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus reticulata; Citrus sinensis; Segregation; Genetic markers.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003001100007
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Skewed RAPD markers in linkage maps of Citrus Genet. Mol. Biol.
Oliveira,Roberto Pedroso de; Aguilar-Vildoso,Carlos Ivan; Cristofani,Mariângela; Machado,Marcos Antônio.
The objective of this work was to analyze the effects of RAPD markers with skewed segregation on genetic linkage maps. Segregation data for 123 Citrus sinensis (L.) Osbeck cv. Pêra markers and 53 C. reticulata Blanco cv. Cravo markers in F1 progeny composed of 94 hybrids were used. Genetic linkage maps of the two varieties were constructed with non-skewed markers (p < 0.05 and p < 0.01) using the program MAPMAKER 3.0 and a pseudo-testcross strategy. The maps were compared to those constructed with all markers. Alterations in the genetic distances were observed based on the location of the skewed markers within the linkage groups. Generally, the skewed markers were located at the end of the linkage groups, sometimes forming entire linkage groups,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic distance; Mendelian segregation; Molecular markers; Segregation distortion.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300021
Registros recuperados: 5
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